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Rat Cluster Of Differentiation 14 (CD14) ELISA Kit

SKU:
RTDL00189
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q63691
Sensitivity:
0.51ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
Monocyte differentiation antigen CD14,Myeloid cell-specific leucine-rich glycoprotein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Rat Cluster Of Differentiation 14 (CD14) ELISA Kit

The Rat Cluster of Differentiation 14 (CD14) ELISA Kit is specifically designed for the accurate and reliable detection of CD14 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with consistent and reproducible results for various research applications.CD14 is a key cell surface marker that plays a crucial role in the immune response by recognizing bacterial lipopolysaccharides and initiating the innate immune system.

It is involved in various inflammatory processes and immune responses, making it a valuable biomarker for studying infections, autoimmune disorders, and inflammatory conditions in rats.With its easy-to-use format and excellent performance characteristics, the Rat CD14 ELISA Kit is an essential tool for researchers in immunology, microbiology, and inflammation-related fields looking to study CD14 expression levels in rat models.

Product Name:Rat Cluster Of Differentiation 14 (CD14) ELISA Kit
Product Code:RTDL00189
Size:96 Assays
Target:CD14
Synonyms:Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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