The Rat CLDN5 (Claudin-5) ELISA Kit is specifically designed for the precise measurement of CLDN5 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Claudin-5 is an integral membrane protein that plays a crucial role in the formation of tight junctions in endothelial cells, regulating paracellular permeability. Dysfunction of CLDN5 has been linked to various pathological conditions, including blood-brain barrier disruption, inflammatory diseases, and cancer metastasis.
Therefore, measuring CLDN5 levels can provide valuable insights into these diseases and aid in the development of targeted therapies. This ELISA kit is a valuable tool for researchers exploring the role of CLDN5 in health and disease, offering reliable and reproducible data for further investigation.
Product Name:
Rat CLDN5 (Claudin-5) ELISA kit
SKU:
RTES01118
Target:
Rat CLDN5 (Claudin-5) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CLDN5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CLDN5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CLDN5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CLDN5. You can calculate the concentration of Rat CLDN5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
85-100
87-101
Average (%)
105
91
94
1:4
Range (%)
90-102
86-101
85-99
Average (%)
97
92
92
1:8
Range (%)
86-100
81-93
84-96
Average (%)
93
86
89
1:16
Range (%)
89-102
81-93
87-99
Average (%)
95
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.49
3.72
0.59
1.35
3.46
Standard deviation
0.03
0.09
0.2
0.04
0.06
0.16
C V (%)
5.56
6.04
5.38
6.78
4.44
4.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CLDN5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CLDN5 in samples. No significant cross-reactivity or interference between Rat CLDN5 and analogues was observed.
