The Rat Clara Cell Protein 16 (CC16) ELISA Kit is specifically designed for the precise measurement of CC16 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for various research applications.CC16, also known as Club Cell Secretory Protein, is a key biomarker for respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and lung injury. As a protein produced by Clara cells in the lungs, CC16 plays a crucial role in protecting the respiratory tract and has been linked to inflammatory responses and oxidative stress.
By measuring CC16 levels, researchers can gain valuable insights into lung health and disease progression, ultimately leading to the development of targeted therapies and interventions. Overall, the Rat Clara Cell Protein 16 (CC16) ELISA Kit is an essential tool for investigating respiratory conditions and advancing our understanding of lung biology.
Product Name:
Rat Clara Cell Protein 16 (CC16) ELISA Kit
Product Code:
RTDL00184
Size:
96 Assays
Target:
CC16
Synonyms:
SCGB1A1, CC10, CCSP, UGB, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein, Secretoglobin Family 1A Member 1, Clara-Cell Specific 10-kD Protein
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
