The Rat CKM (Creatine Kinase, Muscle) ELISA Kit is a cutting-edge tool designed to quantitatively measure the levels of CKM in various biological samples of rats. Creatine kinase, particularly the muscle isoform CKM, is a key enzyme predominantly found in skeletal muscle tissues where it plays a fundamental role in the generation of cellular energy through the conversion of creatine and ATP. This ELISA kit empowers researchers to precisely quantify CKM levels, providing valuable insights into muscle metabolism, cellular energy regulation, and the impact of CKM dysregulation on various physiological processes. CKM serves as a vital component in muscle function, highlighting its importance in energy metabolism and muscle contraction. By utilizing this ELISA kit, researchers can unravel the intricate interplay between CKM levels and muscle-related conditions, exercise physiology, and disease states impacting muscle health.
Accurate measurement of CKM with this kit significantly aids in understanding the role of creatine kinase in muscle energy homeostasis and exercise-induced adaptations. The Rat CKM (Creatine Kinase, Muscle) ELISA Kit by Assay Genie guarantees exceptional sensitivity and specificity, enabling researchers to obtain reliable and reproducible results with high accuracy. Engineered with stringent quality control measures, this kit delivers robust performance and user-friendly protocols, making it the ideal choice for investigating muscle metabolism, energy utilization, and the physiological functions of CKM in research settings.
Product Name:
Rat CKM (Creatine Kinase, Muscle) ELISA Kit
SKU:
AEES00370
Target:
Rat CKM (Creatine Kinase, Muscle)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKM. You can calculate the concentration of Rat CKM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
86-97
88-101
Average (%)
93
92
93
1:4
Range (%)
86-98
81-94
84-96
Average (%)
92
87
89
1:8
Range (%)
92-106
82-95
82-93
Average (%)
99
87
88
1:16
Range (%)
92-105
86-100
86-98
Average (%)
98
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.22
9.46
33.64
5.38
8.54
34.45
Standard deviation
0.29
0.54
1.55
0.31
0.4
1.65
C V (%)
5.56
5.71
4.61
5.76
4.68
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CKM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CKM in samples. No significant cross-reactivity or interference between Rat CKM and analogues was observed.
