Rat CKLFSF6 (Chemokine Like Factor Superfamily 6) CLIA Kit
The Rat CKLFSF6 (Chemokine-Like Factor Superfamily 6) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CKLFSF6 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.CKLFSF6 is a member of the chemokine-like factor superfamily, with roles in immune responses and inflammation.
It plays a critical role in regulating the immune system and has been implicated in various disease states, including cancer and autoimmune disorders. This CLIA kit is essential for the study of CKLFSF6 and its potential therapeutic implications.
Product Name:
Rat CKLFSF6 (Chemokine Like Factor Superfamily 6) CLIA Kit
SKU:
RTES00113
Target:
Rat CKLFSF6 (Chemokine Like Factor Superfamily 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cklfsf6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cklfsf6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cklfsf6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat cklfsf6. You can calculate the concentration of Rat cklfsf6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
85-98
98-112
Average (%)
105
92
105
1:4
Range (%)
93-107
96-107
86-102
Average (%)
99
101
93
1:8
Range (%)
98-111
94-109
86-98
Average (%)
105
100
91
1:16
Range (%)
83-96
101-115
91-104
Average (%)
90
108
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.02
83.36
374.14
51.47
87.14
355.02
Standard deviation
5.31
9.74
38.61
5.98
8.79
22.4
C V (%)
9.83
11.68
10.32
11.62
10.09
6.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat cklfsf6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat cklfsf6 in samples. No significant cross-reactivity or interference between Rat cklfsf6 and analogues was observed.
