Rat CKLFSF5 (Chemokine Like Factor Superfamily 5) CLIA Kit
The Rat CKLFSF5 (Chemokine-like Factor Superfamily 5) CLIA Kit is a cutting-edge assay designed for the precise measurement of CKLFSF5 levels in rat serum, plasma, and tissue homogenates. This advanced kit offers superior sensitivity and specificity, ensuring dependable and consistent results for various research applications.CKLFSF5 is a key molecule implicated in immune response regulation and inflammation, making it a critical player in diseases such as cancer, autoimmune disorders, and infectious diseases.
By accurately quantifying CKLFSF5 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. Don't miss out on the opportunity to advance your research with the Rat CKLFSF5 CLIA Kit from AssayGenie. Order yours today and unlock new possibilities in your studies.
Product Name:
Rat CKLFSF5 (Chemokine Like Factor Superfamily 5) CLIA Kit
SKU:
RTES00112
Target:
Rat CKLFSF5 (Chemokine Like Factor Superfamily 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cklfsf5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cklfsf5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cklfsf5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat cklfsf5. You can calculate the concentration of Rat cklfsf5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
93-104
94-104
Average (%)
91
99
99
1:4
Range (%)
89-105
87-100
91-105
Average (%)
97
93
98
1:8
Range (%)
85-98
93-104
96-109
Average (%)
91
99
101
1:16
Range (%)
95-110
98-110
87-100
Average (%)
100
103
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.81
137.38
431.07
50.65
134.34
414.54
Standard deviation
6.86
10.02
43.28
4.41
15.27
27.15
C V (%)
12.52
7.29
10.04
8.71
11.37
6.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat cklfsf5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat cklfsf5 in samples. No significant cross-reactivity or interference between Rat cklfsf5 and analogues was observed.
