Rat CKLFSF4 (Chemokine Like Factor Superfamily 4) CLIA Kit
The Rat CKLFSF4 (Chemokine-like Factor Superfamily 4) CLIA Kit is a powerful tool for the quantitative detection of CKLFSF4 levels in rat samples such as serum, plasma, and tissue homogenates. This CLIA kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.CKLFSF4, a member of the chemokine-like factor superfamily, plays a critical role in immune regulation and inflammation. Its dysregulation has been implicated in various disease pathologies, including cancer, autoimmune disorders, and infectious diseases.
By accurately measuring CKLFSF4 levels, researchers can gain valuable insights into the role of this protein in disease progression and potentially identify novel therapeutic targets.With its high-quality components and user-friendly protocol, the Rat CKLFSF4 CLIA Kit is a valuable tool for any researcher interested in studying the role of CKLFSF4 in health and disease.
Product Name:
Rat CKLFSF4 (Chemokine Like Factor Superfamily 4) CLIA Kit
SKU:
RTES00111
Target:
Rat CKLFSF4 (Chemokine Like Factor Superfamily 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cklfsf4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cklfsf4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cklfsf4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat cklfsf4. You can calculate the concentration of Rat cklfsf4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
88-103
85-96
Average (%)
98
94
91
1:4
Range (%)
90-105
91-107
96-113
Average (%)
96
98
104
1:8
Range (%)
97-114
86-96
101-115
Average (%)
104
91
108
1:16
Range (%)
91-102
88-102
99-115
Average (%)
96
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
100
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.64
127.91
403.8
52.57
135.97
376.33
Standard deviation
6.54
11.73
32.1
5.12
15.77
38.69
C V (%)
12.66
9.17
7.95
9.74
11.6
10.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat cklfsf4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat cklfsf4 in samples. No significant cross-reactivity or interference between Rat cklfsf4 and analogues was observed.
