The Rat CKLF (Chemokine-Like Factor) CLIA Kit is specifically designed for the quantitative detection of CKLF levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CKLF, a chemokine-like factor, plays a key role in immune responses and inflammation, making it a valuable biomarker for studying diseases and conditions related to these processes.
By accurately measuring CKLF levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop novel therapeutic strategies.Overall, the Rat CKLF CLIA Kit is an essential tool for researchers studying immune responses, inflammation, and related diseases in rat models. Its reliability and precision make it a valuable asset in advancing our understanding of CKLF's role in health and disease.
Product Name:
Rat CKLF (Chemokine Like Factor) CLIA Kit
SKU:
RTES00110
Target:
Rat CKLF (Chemokine Like Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CKLF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKLF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKLF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CKLF. You can calculate the concentration of Rat CKLF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
94-104
103-115
Average (%)
90
99
109
1:4
Range (%)
92-104
97-115
86-98
Average (%)
98
105
93
1:8
Range (%)
90-105
96-111
91-106
Average (%)
97
104
99
1:16
Range (%)
99-116
91-108
102-115
Average (%)
106
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.64
24.55
95.61
10.37
25.74
100.76
Standard deviation
1.24
2.37
7.63
1.04
2.42
7.12
C V (%)
11.65
9.65
7.98
10.03
9.4
7.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CKLF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CKLF in samples. No significant cross-reactivity or interference between Rat CKLF and analogues was observed.
