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Rat Chymase 1, Mast Cell (CMA1) ELISA Kit

SKU:
RTDL00221
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P50339
Sensitivity:
0.57ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CYH,CYM,Alpha-chymase,Mast cell protease I
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Chymase 1, Mast Cell (CMA1) ELISA Kit

The Rat Chymase-1 (Mast Cell CMA1) ELISA Kit is specifically designed for the precise measurement of rat chymase-1 levels in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.Chymase-1 is an important enzyme primarily found in mast cells, playing a crucial role in inflammatory and immune responses. Its involvement in diseases such as asthma, cardiovascular disorders, and dermatitis makes it a key target for research and potential therapeutic interventions.

By accurately measuring chymase-1 levels, researchers can gain valuable insights into its biological functions and its impact on various disease processes. The Rat Chymase-1 ELISA Kit is a reliable and effective tool for studying chymase-1 biology and its implications in different pathological conditions.

Product Name:Rat Chymase 1, Mast Cell (CMA1) ELISA Kit
Product Code:RTDL00221
Size:96 Assays
Target:CMA1
Synonyms:CYH, CYM, Alpha-chymase, Mast cell protease I
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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