The Rat/Chicken Cort Corticosterone ELISA Kit from Assay Genie is a powerful tool for accurately measuring corticosterone levels in rat and chicken samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Corticosterone is a critical hormone involved in stress response, metabolism, and immune function in animals. Monitoring corticosterone levels can provide valuable insight into the physiological and behavioral responses of rats and chickens to various stimuli, making it an indispensable tool for researchers studying stress, endocrine function, or animal models of disease.
With the Rat/Chicken Cort Corticosterone ELISA Kit, researchers can confidently measure corticosterone levels in serum, plasma, and other sample types with ease. This kit is user-friendly and provides rapid results, making it an ideal choice for both experienced researchers and novices alike. Unlock the potential of your research and gain valuable insights with the Rat/Chicken Cort Corticosterone ELISA Kit from Assay Genie.
Product Name:
Rat/Chicken CORT (Corticosterone) ELISA Kit
SKU:
RTES01207
Target:
Rat/Chicken CORT (Corticosterone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.24 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
80-90
88-101
85-106
Average (%)
85
96
94
1:4
Range (%)
82-94
87-94
80-101
Average (%)
88
91
92
1:8
Range (%)
84-100
82-89
83-96
Average (%)
93
86
88
1:16
Range (%)
87-105
89-104
84-99
Average (%)
91
99
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
81-90
85
EDTA plasma (n=5)
86-97
89
Cell culture media (n=5)
85-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
30.38
101.43
206.56
31.45
102.78
208.75
Standard deviation
2.66
7.77
14.56
2.91
8.82
18.02
C V (%)
8.77
7.66
7.05
9.24
8.58
8.63
Sample type &Sample volume:
serum, plasma, urine; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of CORT concentrations in serum, plasma, urine.
Specificity:
This kit recognizes CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed.
