Rat CGRP1 (Calcitonin Gene Related Peptide 1) ELISA Kit
The Rat CGRP1 (Calcitonin Gene-Related Peptide 1) ELISA Kit is a cutting-edge assay meticulously crafted for the precise measurement of CGRP1 levels in various rat biological samples.
CGRP1, a potent neuropeptide well-known for its diverse physiological roles, holds significant significance in the regulation of vascular tone, nociception, neurogenic inflammation, and neurotransmission. As a key player in the sensory nervous system and the management of pain responses, CGRP1 emerges as a critical molecule in understanding neurovascular interactions, migraine pathophysiology, and pain modulation. This ELISA kit, provided by Assay Genie, delivers unparalleled sensitivity and specificity, ensuring accurate and reproducible quantification of CGRP1 levels. Engineered under strict quality assurance measures, this kit ensures robust analytical performance and user-friendly application, making it an optimal choice for researchers delving into the intricate roles of CGRP1 in neurophysiology, pain signaling, and therapeutic interventions. Uncover the complexities of CGRP1 function and its implications in neurobiology with this advanced ELISA kit, facilitating groundbreaking discoveries and therapeutic advancements in pain and migraine research.
Product Name:
Rat CGRP1 (Calcitonin Gene Related Peptide 1) ELISA Kit
SKU:
AEES00335
Target:
Rat CGRP1 (Calcitonin Gene Related Peptide 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CGRP1. You can calculate the concentration of Rat CGRP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
98-110
85-99
Average (%)
93
104
91
1:4
Range (%)
94-108
82-94
87-97
Average (%)
99
87
92
1:8
Range (%)
87-103
82-94
85-95
Average (%)
94
87
90
1:16
Range (%)
91-103
82-95
85-97
Average (%)
98
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.62
96.89
346.87
49.73
105.67
368.64
Standard deviation
2.52
4.83
18.42
2.6
4.87
19.28
C V (%)
5.29
4.99
5.31
5.23
4.61
5.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CGRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CGRP1 in samples. No significant cross-reactivity or interference between Rat CGRP1 and analogues was observed.
