The Rat Complement Factor H (CFH) ELISA Kit is a specialized assay designed to quantitatively detect levels of Complement Factor H in rat biological samples. Complement Factor H is a key regulatory protein in the complement system, playing a crucial role in immune response modulation, complement activation, and regulation of inflammation. This ELISA kit provides researchers with a reliable tool to investigate the levels and functions of Complement Factor H in various physiological and pathological contexts. Accurate measurement of Complement Factor H levels is essential for understanding its involvement in immune regulation, complement-mediated responses, and inflammatory processes. The Rat CFH ELISA Kit enables researchers to explore the intricate mechanisms by which Complement Factor H contributes to immune homeostasis, host defense, and autoimmune disorders.
By quantifying Complement Factor H in biological samples with high sensitivity and specificity, this kit empowers researchers to gain valuable insights into the role of this protein in health and disease. The exceptional sensitivity and specificity of the Rat CFH ELISA Kit ensure precise and reproducible results, allowing researchers to conduct in-depth studies on the functions and regulatory pathways of Complement Factor H. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it a valuable asset for research investigations focused on immune modulation and complement system dynamics.
Product Name:
Rat CFH (Complement Factor H) ELISA Kit
SKU:
AEES00526
Target:
Rat CFH (Complement Factor H)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CFH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CFH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CFH. You can calculate the concentration of Rat CFH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
87-93
99-108
Average (%)
100
90
105
1:4
Range (%)
84-97
94-103
91-106
Average (%)
91
97
98
1:8
Range (%)
98-107
91-105
98-111
Average (%)
103
97
105
1:16
Range (%)
93-105
91-99
91-102
Average (%)
99
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-100
97
EDTA plasma (n=5)
89-99
96
Cell culture media (n=5)
86-97
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.83
499.37
1894.62
173.3
472.81
1790.14
Standard deviation
10.69
23.27
101.93
9.6
23.64
79.12
C V (%)
5.46
4.66
5.38
5.54
5.0
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CFH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CFH in samples. No significant cross-reactivity or interference between Rat CFH and analogues was observed.
