The Rat CEA (Carcinoembryonic Antigen) CLIA Kit is a highly sensitive and specific assay designed for the detection of CEA levels in rat serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it an excellent tool for a variety of research applications.CEA is a glycoprotein biomarker commonly associated with various cancers, including colorectal, pancreatic, and lung cancer. Monitoring CEA levels can aid in cancer diagnosis, prognosis, and treatment management, making this kit essential for studying cancer progression and response to therapy in rat models.
With its easy-to-use format and comprehensive instructions, the Rat CEA CLIA Kit is a valuable asset for researchers looking to investigate the role of CEA in cancer biology and explore potential therapeutic strategies targeting this important biomarker.
Product Name:
Rat CEA (Carcinoembryonic Antigen) CLIA Kit
SKU:
RTES00088
Target:
Rat CEA (Carcinoembryonic Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CEA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CEA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CEA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CEA. You can calculate the concentration of Rat CEA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
85-99
101-114
Average (%)
93
91
108
1:4
Range (%)
90-105
93-106
97-114
Average (%)
96
99
105
1:8
Range (%)
86-96
88-104
86-102
Average (%)
91
95
93
1:16
Range (%)
96-106
93-105
88-98
Average (%)
101
98
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-114
104
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.53
277.27
857.62
89.97
295.8
774.72
Standard deviation
8.39
27.37
67.32
8.95
33.93
61.13
C V (%)
9.07
9.87
7.85
9.95
11.47
7.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CEA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CEA in samples. No significant cross-reactivity or interference between Rat CEA and analogues was observed.
