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Rat CDK5 ELISA Kit

SKU:
RTFI00650
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q03114
Sensitivity:
0.188ng/ml
Range:
0.313-20ng/ml
ELISA Type:
Sandwich
Synonyms:
CDK5, Cdkn5, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, Cell division protein kinase 5, Serine, threonine-protein kinase PSSALRE
Reactivity:
Rat
Research Area:
Cell Death
€649
Frequently bought together:

Description

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Rat CDK5 ELISA Kit

The Rat CDK5 ELISA Kit is a reliable tool for quantifying cyclin-dependent kinase 5 (CDK5) levels in rat samples, including serum, plasma, and tissue homogenates. This kit is known for its high sensitivity and specificity, providing accurate and reproducible results for various research applications.CDK5 is a key enzyme that regulates neuronal development, synaptic plasticity, and cell migration in the central nervous system. Dysregulation of CDK5 has been implicated in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis.

Therefore, measuring CDK5 levels can help researchers better understand the pathophysiology of these diseases and identify potential therapeutic targets.By using the Rat CDK5 ELISA Kit, researchers can effectively study the role of CDK5 in neurodegenerative disorders and explore novel treatment strategies aimed at restoring CDK5 activity. This kit offers a convenient and efficient way to measure CDK5 levels in rat samples, making it a valuable tool for advancing research in the field of neuroscience.

Product Name:Rat CDK5 (Cyclin Dependent Kinase 5) ELISA Kit
Product Code:RTFI00650
Size:96 Assays
Target:Rat CDK5
Alias:CDK5, Cdkn5, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, Cell division protein kinase 5, Serine, threonine-protein kinase PSSALRE
Reactivity:Rat
Detection Method:Sandwich ELISA, Double Antibody
Sensitivity:0.188ng/ml
Range:0.313-20ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Rat CDK5 and the recovery rates were calculated by comparing the measured value to the expected amount of Rat CDK5 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)88-10196
EDTA plasma(n=5)85-10496
UFH plasma(n=5)87-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat CDK5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)86-100%85-104%90-105%
EDTA plasma(n=5)83-91%84-94%82-101%
UFH plasma(n=5)83-98%80-98%82-95%
Intra-Assay:CV <8%
Inter-Assay:CV <10%
Uniprot:Q03114
UniProt Protein Function:CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, CMGC; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; CDK family; CDK5 subfamily; CDK/CDK5 subfamily

Cellular Component: axon; cell junction; cell soma; cyclin-dependent protein kinase 5 activator complex; cytoplasm; cytoskeleton; cytosol; dendrite; filopodium; growth cone; lamellipodium; membrane; neuromuscular junction; nucleolus; nucleus; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function:acetylcholine receptor activator activity; ATP binding; cyclin-dependent protein kinase activity; cytoskeletal protein binding; ephrin receptor binding; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: apoptosis; associative learning; axon extension; axonogenesis; behavioral response to cocaine; cell division; cell migration; cell-matrix adhesion; central nervous system development; central nervous system neuron development; cerebellar cortex development; cerebellar cortex formation; cerebellum development; cerebral cortex development; corpus callosum development; cortical actin cytoskeleton organization and biogenesis; dendrite morphogenesis; exocytosis; forebrain development; hippocampus development; intracellular protein transport; layer formation in the cerebral cortex; motor axon guidance; negative regulation of axon extension; negative regulation of cell cycle; negative regulation of protein export from nucleus; negative regulation of protein ubiquitination; negative regulation of proteolysis; negative regulation of synaptic plasticity; negative regulation of transcription, DNA-dependent; neurite development; neurite morphogenesis; neuron apoptosis; neuron differentiation; neuron migration; nucleocytoplasmic transport; oligodendrocyte differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of calcium ion-dependent exocytosis; positive regulation of neuron apoptosis; positive regulation of protein binding; positive regulation of protein kinase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; receptor catabolic process; receptor clustering; regulated secretory pathway; regulation of cell migration; regulation of excitatory postsynaptic membrane potential; regulation of postsynaptic membrane potential; regulation of synaptic plasticity; response to cocaine; response to wounding; rhythmic process; Schwann cell development; sensory perception of pain; serine phosphorylation of STAT3 protein; skeletal muscle development; synaptic transmission, dopaminergic; synaptic transmission, glutamatergic; synaptic vesicle endocytosis; synaptogenesis; telencephalon development; visual learning

NCBI Summary:serine/threonine kinase involved in synaptic regulation and neuronal development; phosphorylates synaptic protein Pctaire1; regulates acetylcholine receptor expression [RGD, Feb 2006]
UniProt Code:Q03114
NCBI GenInfo Identifier:18266682
NCBI Gene ID:140908
NCBI Accession:NP_543161
UniProt Related Accession:Q03114
Molecular Weight:32kDa
NCBI Full Name:cyclin-dependent-like kinase 5
NCBI Synonym Full Names:cyclin-dependent kinase 5
NCBI Official Symbol:Cdk5  
NCBI Protein Information:cyclin-dependent-like kinase 5
UniProt Protein Name:Cyclin-dependent-like kinase 5
UniProt Synonym Protein Names:Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
Protein Family:CDK5RAP1-like protein
UniProt Gene Name:Cdk5  
UniProt Entry Name:CDK5_RAT
StepProcedure
1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3. Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4. Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37°C for 90 min.
6. Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7. Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9. Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10. Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11. Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12. Add 90 µL of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13. Add 50 µL of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum:

If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clotovernight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Removeserum and assay promptly or aliquot and store the samples at-80°C. Avoid multiple freeze-thaw cycles.

Plasma: Collect plasma using EDTA or heparin as an anti-coagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid: Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell Culture Supernatant: Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell Lysates: Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20°C.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenizein 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or-80°C.
Tissue Lysates: Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk: Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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