null

Rat CD8 (Cluster of Differentiation 8) ELISA Kit

SKU:
AEES00353
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.75 ng/mL
Range:
1.25-80 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
Frequently bought together:

Description

Rat CD8 (Cluster of Differentiation 8) ELISA Kit

The Rat CD8 (Cluster of Differentiation 8) ELISA Kit offers a cutting-edge solution for the precise quantitative measurement of CD8 levels in a variety of rat biological samples. CD8 is a crucial cell surface glycoprotein expressed on cytotoxic T cells and plays a pivotal role in immune responses, particularly in the identification and elimination of infected or aberrant cells. The assay is specifically designed to enable researchers to explore the expression levels of CD8, offering valuable insights into cellular immunity, immune responses, and immunopathology. Accurate quantification of CD8 is essential for understanding its contributions to host defense mechanisms, immune surveillance, and autoimmune disorders.

Through the meticulous detection of CD8 levels, researchers can unravel the complexities of T cell-mediated immunity and investigate the role of CD8+ T cells in various disease processes. The Rat CD8 ELISA Kit by Assay Genie is meticulously developed to provide exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance, making it an indispensable tool for researchers investigating the functions and implications of CD8 in immunology and disease pathogenesis.

Product Name:Rat CD8 (Cluster of Differentiation 8) ELISA Kit
Product Code:AEES00353
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:1.25-80 ng/mL
Sensitivity:0.75 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat CD8 in samples. No significant cross-reactivity or interference between Rat CD8 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat CD8 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CD8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CD8. You can calculate the concentration of Rat CD8 in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CD8 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CD8 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)4.4412.7333.174.1213.5530.74
Standard deviation0.300.661.380.220.631.62
CV (%)6.765.184.165.344.655.27

Recovery

The recovery of Rat CD8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)85-9891
EDTA plasma (n=8)88-9994
Cell culture media (n=8)93-10498
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

Related Products