The Rat CASP9 (Caspase 9) ELISA Kit is a specialized assay designed for the quantitative detection of Caspase 9 levels in rat biological samples.
Caspase 9 is a critical enzyme involved in programmed cell death, also known as apoptosis, playing a key role in regulating cell survival and proliferation. Accurate measurement of Caspase 9 levels is essential for understanding its role in apoptotic pathways, regulatory mechanisms in cell death, and its implications in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. The ELISA kit provides a reliable and precise method to quantify Caspase 9 concentrations in biological samples, enabling researchers to investigate its involvement in various cellular processes and disease states. Manufactured under stringent quality control standards, the Rat CASP9 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Its user-friendly protocols, robust performance, and accurate measurements make it a valuable tool for researchers studying apoptosis, cell signaling pathways, and therapeutic strategies targeting Caspase 9 in various research fields.
Product Name:
Rat CASP9 (Caspase 9) ELISA Kit
SKU:
AEES00345
Target:
Rat CASP9 (Caspase 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP9. You can calculate the concentration of Rat CASP9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
98-111
96-113
Average (%)
97
104
103
1:4
Range (%)
91-107
81-94
83-98
Average (%)
99
86
89
1:8
Range (%)
90-105
81-95
81-92
Average (%)
97
88
87
1:16
Range (%)
88-101
82-92
86-98
Average (%)
95
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.89
12.56
37.09
4.75
13.8
37.03
Standard deviation
0.3
0.54
1.86
0.25
0.62
1.31
C V (%)
6.13
4.3
5.01
5.26
4.49
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CASP9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP9 in samples. No significant cross-reactivity or interference between Rat CASP9 and analogues was observed.
