The Rat Casp9 (Caspase-9) CLIA Kit is a highly reliable and sensitive tool designed for the accurate detection of caspase-9 levels in rat samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results for their studies.Caspase-9 is a key player in the apoptosis signaling pathway, playing a critical role in programmed cell death. Dysregulation of caspase-9 has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases.
As such, measuring caspase-9 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high quality and performance, the Rat Casp9 CLIA Kit is an essential tool for researchers studying apoptosis, cell death, and various disease processes in rat models. Trust in this kit to deliver accurate and reliable results for your research needs.
Product Name:
Rat CASP9 (Caspase 9) CLIA Kit
SKU:
RTES00098
Target:
Rat CASP9 (Caspase 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASP9. You can calculate the concentration of Rat CASP9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
100-113
103-116
Average (%)
94
107
110
1:4
Range (%)
88-100
85-96
88-102
Average (%)
94
91
93
1:8
Range (%)
95-110
92-106
101-116
Average (%)
103
98
108
1:16
Range (%)
86-97
88-100
90-103
Average (%)
91
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.3
9.16
1.11
2.29
9.34
Standard deviation
0.1
0.27
0.56
0.1
0.18
0.86
C V (%)
9.43
11.74
6.11
9.01
7.86
9.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASP9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP9 in samples. No significant cross-reactivity or interference between Rat CASP9 and analogues was observed.
