The Rat CASP3 (Caspase 3) ELISA Kit is a high-quality assay designed for the quantitative detection of Caspase 3 levels in rat biological samples.
Caspase 3 is a critical enzyme involved in apoptosis (programmed cell death) and plays a significant role in regulating various cellular processes, including cell differentiation, proliferation, and activation of inflammatory pathways. Accurate measurement of Caspase 3 levels can provide insights into apoptotic pathways, cellular response to stress, and the development of various diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. This ELISA kit offers a reliable and precise method to assess Caspase 3 activity, making it an indispensable tool for apoptosis research and drug discovery studies. Built upon stringent quality control standards, Assay Genie's Rat CASP3 ELISA Kit delivers exceptional sensitivity and specificity, ensuring reproducible results with robust performance. With user-friendly protocols and accurate quantification capabilities, this kit is an excellent choice for investigating apoptotic mechanisms and related pathways in research settings.
Product Name:
Rat CASP3 (Caspase 3) ELISA Kit
SKU:
AEES00344
Target:
Rat CASP3 (Caspase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP3. You can calculate the concentration of Rat CASP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
90-105
99-112
Average (%)
106
96
105
1:4
Range (%)
90-106
80-94
84-97
Average (%)
97
86
90
1:8
Range (%)
90-101
84-96
84-97
Average (%)
96
91
91
1:16
Range (%)
87-102
82-96
85-101
Average (%)
94
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
101
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
3.21
9.83
1.05
3.1
9.92
Standard deviation
0.07
0.14
0.54
0.06
0.12
0.49
C V (%)
6.42
4.36
5.49
5.71
3.87
4.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CASP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed.
