The Rat Casp14 (Caspase-14) CLIA Kit is specifically designed for the quantitative detection of caspase-14 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Caspase-14 is an important protein involved in programmed cell death, playing a role in skin barrier function and inflammation.
Dysregulation of caspase-14 has been linked to various skin disorders, making it a valuable biomarker for studying skin health and disease.The Rat Casp14 CLIA Kit provides a convenient and efficient solution for measuring caspase-14 levels in rat samples, allowing researchers to better understand the role of this protein in skin biology and potentially identify new therapeutic targets for skin conditions.
Product Name:
Rat CASP14 (Caspase 14) CLIA Kit
SKU:
RTES00099
Target:
Rat CASP14 (Caspase 14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASP14. You can calculate the concentration of Rat CASP14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
100-116
89-102
Average (%)
96
109
96
1:4
Range (%)
101-119
99-117
100-114
Average (%)
109
107
107
1:8
Range (%)
92-108
96-113
92-107
Average (%)
100
103
98
1:16
Range (%)
92-103
83-96
102-120
Average (%)
97
90
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
191.15
436.6
1384.19
197.49
425.2
1454.66
Standard deviation
22.02
43.44
125.96
22.69
37.67
129.17
C V (%)
11.52
9.95
9.1
11.49
8.86
8.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASP14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP14 in samples. No significant cross-reactivity or interference between Rat CASP14 and analogues was observed.
