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Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit

SKU:
RTDL00170
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11730
Sensitivity:
0.123ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CAMKII,CAMK2G,CAMK,CAMK-II,CAMKG,CaM Kinase,CaM kinase II subunit gamma,CaMK-II subunit gamma
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit

The Rat Calcium/Calmodulin-Dependent Protein Kinase II Gamma (CAMK2G) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection and quantification of CAMK2G levels in rat serum, plasma, and cell culture supernatants. This kit is ideal for researchers studying the role of CAMK2G in various physiological and pathological processes.CAMK2G is a key regulator of neuronal function, playing a crucial role in synaptic plasticity, learning, and memory. Dysregulation of CAMK2G has been implicated in neurological disorders such as Alzheimer's disease and epilepsy.

This ELISA kit provides researchers with a reliable tool to study the expression and activity of CAMK2G, aiding in the development of novel therapeutic strategies.With its high sensitivity and reproducibility, the Rat CAMK2G ELISA Kit from AssayGenie is a valuable tool for researchers investigating the molecular mechanisms underlying neurological disorders and exploring potential therapeutic targets.

Product Name:Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit
Product Code:RTDL00170
Size:96 Assays
Target:CAMK2g
Synonyms:CAMKII, CAMK2G, CAMK, CAMK-II, CAMKG, CaM Kinase, CaM kinase II subunit gamma, CaMK-II subunit gamma
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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