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Rat Cadherin, Epithelial (CDHE) ELISA Kit

SKU:
RTDL00194
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9R0T4
Sensitivity:
0.079ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD324,CDH1,Arc1,ECAD,CDH-E,E-CAD,LCAM,UVO,Cadherin 1 Type 1,Uvomorulin,E-Cadherin,Calcium-Dependent Adhesion Protein, Epithelial,Liver Cell Adhesion Molecule
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Cadherin, Epithelial (CDHE) ELISA Kit

The Rat Cadherin Epithelial (CDHE) ELISA Kit is specifically designed for the precise measurement of CDHE levels in rat serum, plasma, and cell culture samples. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it perfect for various research applications. CDHE is a vital cell adhesion molecule that plays a critical role in maintaining the integrity of epithelial tissues. Dysregulation of CDHE expression has been linked to various diseases, including cancer and inflammatory disorders, making it an important biomarker for studying these conditions and exploring therapeutic interventions.

This ELISA kit offers researchers a valuable tool for investigating the function and regulation of CDHE in biological processes, ultimately advancing our understanding of epithelial cell biology and potential treatment options for related diseases.

Product Name:Rat Cadherin, Epithelial (CDHE) ELISA Kit
Product Code:RTDL00194
Size:96 Assays
Target:CDHE
Synonyms:CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule
Detection Method:Sandwich
Range:0.312-20 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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