The Rat CA1 (Carbonic Anhydrase I) CLIA Kit is an innovative assay kit designed for the quantitative measurement of CA1 levels in rat samples. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for research purposes.Carbonic Anhydrase I is a key enzyme involved in the regulation of pH in various tissues and organs, playing a crucial role in processes such as respiration, calcification, and acid-base balance. Abnormal levels of CA1 have been linked to various diseases and conditions, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.
The Rat CA1 CLIA Kit is user-friendly and efficient, providing researchers with a powerful tool for investigating the role of CA1 in health and disease. With its advanced technology and precise performance, this kit is a valuable asset for any laboratory conducting research on carbonic anhydrase I in rat models.
Product Name:
Rat CA1 (Carbonic Anhydrase I) CLIA Kit
SKU:
RTES00086
Target:
Rat CA1 (Carbonic Anhydrase I)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CA1. You can calculate the concentration of Rat CA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
85-99
93-110
Average (%)
91
91
100
1:4
Range (%)
86-100
99-114
92-110
Average (%)
92
105
100
1:8
Range (%)
94-107
87-98
97-108
Average (%)
101
93
102
1:16
Range (%)
91-107
84-96
96-112
Average (%)
99
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
101-114
107
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.39
3.87
0.43
1.33
3.76
Standard deviation
0.05
0.15
0.29
0.04
0.1
0.27
C V (%)
10.87
10.79
7.49
9.3
7.52
7.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CA1 in samples. No significant cross-reactivity or interference between Rat CA1 and analogues was observed.
