The Rat Complement Component 5a (C5a) ELISA Kit is a specialized assay developed to accurately quantify levels of C5a within rat biological samples. Complement Component 5a, a potent pro-inflammatory peptide, plays a crucial role in the activation of the complement system, regulation of immune responses, and modulation of inflammatory processes. This ELISA kit provides researchers with a precise tool to investigate the levels and functions of C5a in various physiological and pathological conditions. Accurate measurement of C5a levels is essential for understanding its involvement in immune system activation, complement-mediated pathways, and inflammatory responses. The Rat C5a ELISA Kit enables researchers to explore the complex mechanisms by which C5a influences immune cell recruitment, cytokine production, and inflammatory cascades.
By quantifying C5a in biological samples with high sensitivity and specificity, this kit empowers researchers to uncover valuable insights into the role of C5a in inflammation, infection, and autoimmune disorders. The exceptional sensitivity and specificity of the Rat C5a ELISA Kit ensures accurate and reproducible results, allowing researchers to conduct detailed investigations into the functions and regulatory pathways of C5a. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an ideal choice for research studies focusing on immune modulation, complement system activation, and inflammatory signaling pathways.
Product Name:
Rat C5a (Complement Component 5a) ELISA Kit
SKU:
AEES00527
Target:
Rat C5a (Complement Component 5a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C5a. You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-110
94-104
Average (%)
95
105
98
1:4
Range (%)
84-100
86-102
96-108
Average (%)
92
94
101
1:8
Range (%)
98-110
101-109
88-101
Average (%)
103
105
94
1:16
Range (%)
82-98
87-98
94-106
Average (%)
90
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.08
113.3
497.15
45.05
120.13
443.23
Standard deviation
2.46
5.35
26.4
2.58
5.93
22.69
C V (%)
5.58
4.72
5.31
5.73
4.94
5.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat C5a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C5a in samples. No significant cross-reactivity or interference between Rat C5a and analogues was observed.
