Rat C4a (Complement Component 4a) ELISA Kit (AEES00367)
- SKU:
- AEES00367
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.19 ng/mL
- Range:
- 0.31-20 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat C4a (Complement Component 4a) ELISA Kit
The Rat C4a (Complement Component 4a) ELISA Kit serves as a specialized tool for the accurate quantitative measurement of Complement Component 4a levels specifically in rat-derived biological samples. Complement Component 4a, a crucial fragment involved in the complement system cascade, contributes significantly to inflammation, immune responses, and host defense mechanisms. This ELISA kit enables researchers to precisely quantify C4a levels, facilitating the investigation of immune regulation, inflammatory processes, and complement system activation in various biological contexts. C4a plays a pivotal role in various immune and inflammatory responses, and its quantitative assessment is instrumental in understanding the intricate mechanisms underlying these pathways. Through the precise measurement of C4a, researchers can gain valuable insights into the role of this complement component in health and disease, providing a foundation for targeted therapeutic strategies, disease interventions, and immunological studies.
The Rat C4a ELISA Kit from Assay Genie offers remarkable sensitivity and specificity, ensuring accurate and reproducible results. By adhering to stringent quality control standards, this kit delivers robust performance and reliable data, all while maintaining user-friendly protocols. As a valuable research tool, this ELISA kit enables scientists to delve deeper into the complexities of the complement system and its implications for diverse biological processes.
| Product Name: | Rat C4a (Complement Component 4a) ELISA Kit |
| Product Code: | AEES00367 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat C4a in samples. No significant cross-reactivity or interference between Rat C4a and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat C4a concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C4a. You can calculate the concentration of Rat C4a in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
Related Products
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