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Rat C4 Binding Protein Alpha (C4BPa) ELISA Kit

SKU:
RTDL00160
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q63514
Sensitivity:
25.6pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
C4BP-a,Complement Component 4 Binding Protein,Alpha
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

Rat C4 Binding Protein Alpha (C4BPa) ELISA Kit

The Rat C4 Binding Protein Alpha (C4BPA) ELISA Kit is a reliable and accurate tool for detecting C4BPA levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results, making it suitable for various research applications.C4BPA is a key protein involved in the regulation of the complement system and immune response. It plays a crucial role in inflammation, infection, and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing treatment strategies.

With the Rat C4BPA ELISA Kit, researchers can quickly and easily measure C4BPA levels in rat samples, providing valuable insights into the role of this protein in health and disease. It is an essential tool for advancing our understanding of the immune system and developing new therapeutic approaches.

Product Name:Rat C4 Binding Protein Alpha (C4BPa) ELISA Kit
Product Code:RTDL00160
Size:96 Assays
Target:C4BPa
Synonyms:C4BP-a, Complement Component 4 Binding Protein,Alpha
Detection Method:Sandwich
Range:62.5-4000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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