The Rat C3a (Complement Component 3a) ELISA Kit is a specialized assay designed for the precise quantification of Complement Component 3a levels in a variety of biological samples obtained from rat models.
Complement Component 3a is a pivotal protein fragment generated during the activation of the complement system, playing a critical role in inflammation, immunity, and host defense. Accurate measurement of C3a levels is essential for investigating immune responses, inflammatory pathways, and complement system activities. This ELISA kit offers researchers a reliable and high-throughput method to quantify Complement C3a, enabling in-depth exploration of its functions, modulation, and involvement in various pathophysiological processes. By quantitatively assessing C3a, researchers can gain valuable insights into the intricate interplay between the complement system and immune responses, shedding light on its implications for health and disease. The Rat C3a ELISA Kit by Assay Genie is characterized by its exceptional sensitivity and specificity, ensuring the generation of precise and repeatable results. Manufactured under strict quality control standards, this kit guarantees robust and dependable performance, combined with user-friendly protocols, making it an ideal instrument for cutting-edge research endeavors focused on unraveling the complexities of the complement system and its impact on immune regulation.
Product Name:
Rat C3a (Complement Component 3a) ELISA Kit
SKU:
AEES00366
Target:
Rat C3a (Complement Component 3a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C3a. You can calculate the concentration of Rat C3a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
95-109
86-101
Average (%)
92
100
93
1:4
Range (%)
86-100
87-98
85-97
Average (%)
93
92
91
1:8
Range (%)
88-99
85-100
88-102
Average (%)
94
91
94
1:16
Range (%)
89-100
82-96
83-96
Average (%)
95
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.91
4.43
17.14
1.78
4.78
16.69
Standard deviation
0.11
0.22
0.75
0.09
0.21
0.71
C V (%)
5.76
4.97
4.38
5.06
4.39
4.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat C3a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C3a in samples. No significant cross-reactivity or interference between Rat C3a and analogues was observed.
