The Rat C3 (Complement Component 3) ELISA Kit is a cutting-edge assay tailored for the precise quantitation of Complement Component 3 levels within diverse biological samples collected from rat models. Complement Component 3 is a pivotal protein in the complement system, crucial for immune responses, pathogen clearance, and inflammatory processes.
Accurate measurement of C3 levels is instrumental in elucidating the functionality of the complement system, evaluating immune activities, and deciphering the role of C3 in various disease conditions. This ELISA kit empowers researchers to explore the intricate dynamics of Complement Component 3, shedding light on its involvement in immune regulation, infectious diseases, autoimmune disorders, and inflammatory responses. By quantifying C3 levels, researchers can gain valuable insights into the immune system's functionality, disease pathogenesis, and potential therapeutic targets. Understanding the quantitative variations of C3 is fundamental in deciphering immune signaling pathways and developing targeted interventions for immune-related disorders. The Rat C3 ELISA Kit offered by Assay Genie is characterized by exceptional sensitivity, offering precise and reproducible quantification of C3 levels in rat samples. Manufactured under rigorous quality control standards, this kit ensures robust performance and user-friendly protocols, making it an indispensable tool for researchers investigating the intricate interplay between the complement system and immune responses in health and disease.
Product Name:
Rat C3 (Complement Component 3) ELISA Kit
SKU:
AEES00364
Target:
Rat C3 (Complement Component 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C3. You can calculate the concentration of Rat C3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
95-109
97-111
Average (%)
102
102
104
1:4
Range (%)
92-102
81-94
83-95
Average (%)
97
86
88
1:8
Range (%)
88-99
85-98
84-94
Average (%)
93
91
88
1:16
Range (%)
87-99
82-97
82-97
Average (%)
93
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.32
72.35
248.74
26.86
77.94
256.58
Standard deviation
1.54
4.27
8.28
1.85
4.21
10.37
C V (%)
6.08
5.9
3.33
6.89
5.4
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat C3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C3 in samples. No significant cross-reactivity or interference between Rat C3 and analogues was observed.
