The Rat C-P C-Peptide CLIA Kit is a cutting-edge assay designed for the precise measurement of C-peptide levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.C-peptide is a key marker for assessing insulin secretion and monitoring pancreatic function in diabetes and metabolic disorders. Its levels can provide valuable insights into insulin production, beta-cell activity, and glucose metabolism, making it a crucial biomarker for studying diabetes and related complications.
With its advanced technology and reliable performance, the Rat C-P C-Peptide CLIA Kit is an indispensable tool for researchers investigating the mechanisms of diabetes, exploring potential therapeutic interventions, and advancing our understanding of metabolic diseases.
Product Name:
Rat C-P (C-peptide) CLIA Kit
SKU:
RTES00024
Target:
Rat C-P (C-peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat C-P. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat C-P. You can calculate the concentration of Rat C-P in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
91-104
86-98
Average (%)
100
96
91
1:4
Range (%)
91-102
85-97
102-116
Average (%)
97
91
110
1:8
Range (%)
100-116
92-107
87-100
Average (%)
108
100
93
1:16
Range (%)
85-97
86-97
98-112
Average (%)
90
91
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
99-112
104
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
304.2
613.6
2112.25
302.4
653.81
2238.05
Standard deviation
32.7
48.84
168.35
28.94
77.54
175.91
C V (%)
10.75
7.96
7.97
9.57
11.86
7.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat C-P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C-P in samples. No significant cross-reactivity or interference between Rat C-P and analogues was observed.
