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Rat BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) ELISA Kit

SKU:
AEES00333
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
56.25 pg/mL
Range:
93.75-6000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) ELISA Kit

The Rat BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) ELISA Kit is a cutting-edge assay meticulously designed for the quantification of the chemokine BRAK/CXCL14 in various rat biological samples. BRAK, a unique chemokine primarily expressed in breast and kidney tissues, plays a significant role in modulating immune responses, cell migration, and inflammation. With its distinctive expression pattern and functions, BRAK/CXCL14 serves as a critical factor in diverse physiological processes, making it an intriguing target for research exploring immune regulation, cell signaling, and disease pathogenesis. This ELISA kit from Assay Genie is engineered to deliver exceptional sensitivity and specificity, ensuring accurate and reproducible measurement of BRAK/CXCL14 levels.

Manufactured under strict quality control measures, this kit offers robust performance and simplicity of use, making it an ideal choice for researchers investigating the multifaceted roles of BRAK/CXCL14 in biological processes and disease contexts. Unlock the intricate functions of BRAK/CXCL14 with this advanced ELISA kit, paving the way for enhanced understanding and therapeutic development in the field of chemokine biology.

Product Name:Rat BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) ELISA Kit
Product Code:AEES00333
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:93.75-6000 pg/mL
Sensitivity:56.25 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Rat BRAK/CXCL14 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat BRAK/CXCL14 concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BRAK/CXCL14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BRAK/CXCL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BRAK/CXCL14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BRAK/CXCL14. You can calculate the concentration of Rat BRAK/CXCL14 in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BRAK/CXCL14 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BRAK/CXCL14 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)284.93882.582799.87272.12851.482803.97
Standard deviation15.3644.2287.9217.5038.49132.07
CV (%)5.395.013.146.434.524.71

Recovery

The recovery of Rat BRAK/CXCL14 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)90-10798
EDTA plasma (n=8)88-10395
Cell culture media (n=8)91-10699
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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