Rat BID (BH3 Interacting Domain Death Agonist) CLIA Kit
The Rat BID (BH3 Interacting Domain Death Agonist) CLIA Kit is a powerful tool for the precise measurement of rat BID levels in various biological samples, including serum, plasma, and tissue lysates. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and dependable results for a wide range of research applications.BID is a key player in the regulation of apoptosis, or programmed cell death, and is involved in various cellular processes such as mitochondrial dynamics and cell survival.
Dysregulation of BID has been linked to several diseases, including cancer and neurodegenerative disorders, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.With the Rat BID CLIA Kit, researchers can confidently analyze BID levels and gain deeper insights into its role in physiological and pathological processes. Trust in the reliability and precision of this kit for your research needs.
Product Name:
Rat BID (BH3 Interacting Domain Death Agonist) CLIA Kit
SKU:
RTES00063
Target:
Rat BID (BH3 Interacting Domain Death Agonist)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Bid. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Bid and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Bid, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Bid. You can calculate the concentration of Rat Bid in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
90-104
84-100
Average (%)
92
96
91
1:4
Range (%)
87-100
103-115
92-108
Average (%)
94
108
98
1:8
Range (%)
95-106
84-98
95-107
Average (%)
100
91
100
1:16
Range (%)
88-104
88-98
90-106
Average (%)
95
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
100-111
105
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.6
388.58
1663.36
196.33
390.33
1537.44
Standard deviation
20.68
41.58
122.09
17.96
36.81
171.27
C V (%)
10.68
10.7
7.34
9.15
9.43
11.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Bid concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Bid in samples. No significant cross-reactivity or interference between Rat Bid and analogues was observed.
