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Rat Bgn (Biglycan) ELISA Kit

SKU:
AEFI00924
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
4.688pg/ml
Range:
7.813-500pg/ml
ELISA Type:
Sandwich
Reactivity:
Rat
  • Rat Bgn (Biglycan) ELISA Kit
  • Rat Bgn (Biglycan) ELISA Kit
$719
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Description

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Rat Bgn (Biglycan) ELISA Kit

The Rat BGN (Biglycan) ELISA Kit is specifically designed to detect levels of biglycan in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and dependable results for various research applications.Biglycan is a key protein involved in extracellular matrix organization and plays a crucial role in tissue development and repair. Dysregulation of biglycan has been linked to various diseases, including musculoskeletal disorders and cardiovascular diseases.

By measuring biglycan levels, researchers can gain insights into disease progression and potentially identify new therapeutic targets.Overall, the Rat BGN ELISA Kit is a valuable tool for studying biglycan biology and its role in disease pathology, offering researchers a reliable method for measuring biglycan levels in rat samples.

Product Name:Rat Bgn (Biglycan) ELISA Kit
Product Code:AEFI00924
Size:96T
Alias:Biglycan, Bone/cartilage proteoglycan I, PG-S1, Bgn
Detection method:Sandwich ELISA, Double Antibody
Application:Bgn ELISA Kit allows for the in vitro quantitative determination of Bgn concentrations in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:< 4.688pg/ml
Range:7.813-500pg/ml
Storage:2-8°C for 6 months
Note:For Research Use Only
Recovery: Matrices listed below were spiked with certain level of Bgn and the recovery rates were calculated by comparing the measured value to the expected amount of Bgn in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)85-10495
EDTA plasma(n=5)91-9593
heparin plasma(n=5)85-10295
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bgn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)90-103%85-101%87-105%
EDTA plasma(n=5)85-100%83-94%89-95%
heparin plasma(n=5)94-97%80-97%81-96%
CV(%): Intra-Assay: CV<8%
Inter-Assay: CV<10%

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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