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Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

SKU:
AEES00321
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

The Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit is a highly specialized assay designed to quantitatively detect and measure the levels of bFGF/FGF2 in various biological samples from rats.

Basic Fibroblast Growth Factor (bFGF/FGF2) is a crucial growth factor known for its diverse roles in cell proliferation, angiogenesis, wound healing, and tissue regeneration. Its aberrant expression has been associated with several pathological conditions, making accurate measurement of bFGF/FGF2 levels essential for studying its biological functions and potential implications in diseases. This ELISA kit provides exceptional sensitivity and specificity for reliable and reproducible results, allowing researchers to delve deeper into the molecular mechanisms mediated by bFGF/FGF2. By offering robust performance and stringent quality control measures, the Rat bFGF/FGF2 ELISA Kit ensures precise quantification of bFGF/FGF2, thus serving as an indispensable tool for researchers exploring the intricate signaling pathways and therapeutic implications of this growth factor. Trust in the Rat bFGF/FGF2 ELISA Kit to advance your research in cell biology, regenerative medicine, and disease pathogenesis.

Product Name:Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
Product Code:AEES00321
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat bFGF/FGF2 in samples. No significant cross-reactivity or interference between Rat bFGF/FGF2 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat bFGF/FGF2 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat bFGF/FGF2. You can calculate the concentration of Rat bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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