The Rat β-TG (Beta Thromboglobulin) CLIA Kit is designed for the precise measurement of beta thromboglobulin levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Beta thromboglobulin is a key protein involved in platelet activation and aggregation, playing a crucial role in hemostasis and thrombosis. Abnormal levels of beta thromboglobulin have been associated with various cardiovascular diseases and inflammatory conditions, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Rat β-TG CLIA Kit is an essential tool for researchers investigating the role of beta thromboglobulin in physiological and pathological processes related to platelet function and thrombosis in rat models.
Product Name:
Rat beta-TG (beta-Thromboglobulin) CLIA Kit
SKU:
RTES00601
Target:
Rat beta-TG (beta-Thromboglobulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 ng/mL
Detection range:
93.75-6000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat β-TG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat β-TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat β-TG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat β-TG. You can calculate the concentration of Rat β-TG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
86-101
84-98
Average (%)
105
92
90
1:4
Range (%)
96-109
89-103
98-114
Average (%)
103
95
105
1:8
Range (%)
97-111
85-98
91-106
Average (%)
104
91
98
1:16
Range (%)
102-116
91-109
102-113
Average (%)
108
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
273.85
777.33
2948.0
273.68
780.8
3202.11
Standard deviation
29.79
69.57
268.56
31.75
56.3
247.52
C V (%)
10.88
8.95
9.11
11.6
7.21
7.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat β-TG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat β-TG in samples. No significant cross-reactivity or interference between Rat β-TG and analogues was observed.
