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Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

SKU:
RTDL00142
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5I0J6
Sensitivity:
6.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL13,SCYB13,BLC,BCA1,BLR1L,ANGIE,ANGIE2,Chemokine(C-X-C-Motif)ligand 13,Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

The Rat B-Lymphocyte Chemoattractant-1 (BLC1) ELISA Kit is a powerful tool for researchers studying immune responses in rats. This kit allows for the precise measurement of BLC1 levels in rat serum, plasma, and cell culture supernatants with high sensitivity and accuracy.BLC1 is a chemokine that plays a key role in recruiting B-lymphocytes to sites of inflammation and infection. This chemokine is essential for the proper functioning of the immune system and is implicated in various immune-mediated diseases.

By accurately quantifying BLC1 levels, researchers can gain valuable insights into the immune response in rats and potentially identify novel therapeutic targets for immune-related disorders. The Rat BLC1 ELISA Kit provides reliable and reproducible results, making it an ideal choice for a wide range of research applications in immunology and inflammation.

Product Name:Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit
Product Code:RTDL00142
Size:96 Assays
Target:BLC1
Synonyms:CXCL13, SCYB13, BLC, BCA1, BLR1L, ANGIE, ANGIE2, Chemokine(C-X-C-Motif)ligand 13, Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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