The Rat ATXN1 (Ataxin-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of ATXN1 levels in rat samples. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.ATXN1 is a key protein involved in the development of neurodegenerative disorders, including spinocerebellar ataxia type 1 (SCA1).
By measuring ATXN1 levels, researchers can gain valuable insights into the pathogenesis of these disorders and potentially identify new therapeutic targets.Overall, the Rat ATXN1 CLIA Kit offers a powerful tool for studying neurodegenerative diseases and advancing our understanding of ATXN1-related mechanisms.
Product Name:
Rat ATXN1 (Ataxin 1) CLIA Kit
SKU:
RTES00050
Target:
Rat ATXN1 (Ataxin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ATXN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ATXN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ATXN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ATXN1. You can calculate the concentration of Rat ATXN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
98-114
86-98
Average (%)
91
104
92
1:4
Range (%)
87-98
85-101
99-115
Average (%)
93
92
107
1:8
Range (%)
88-103
89-105
98-114
Average (%)
95
96
104
1:16
Range (%)
95-110
102-115
92-105
Average (%)
102
108
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-97
90
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
100-114
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.98
479.72
1712.22
216.5
466.51
1840.05
Standard deviation
21.18
57.18
129.27
25.74
40.4
170.94
C V (%)
9.85
11.92
7.55
11.89
8.66
9.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ATXN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ATXN1 in samples. No significant cross-reactivity or interference between Rat ATXN1 and analogues was observed.
