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Rat ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit

SKU:
RTDL00124
Product Type:
ELISA Kit
Sensitivity:
0.136ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Proton pump beta chain,Gastric H(+)/K(+) ATPase subunit beta
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Rat ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit

The Rat ATPase H+/K+ Exchanging Beta Polypeptide (ATP4B) ELISA Kit is specifically designed for the quantitative detection of ATP4B levels in rat samples including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, resulting in accurate and reproducible results for various research applications.ATP4B is a vital component of the gastric proton pump, responsible for the secretion of hydrochloric acid in the stomach. Dysregulation of ATP4B expression has been linked to gastric disorders such as acid reflux, ulcers, and gastritis.

By measuring ATP4B levels, researchers can gain valuable insights into gastric function and potential therapeutic targets for related conditions.Overall, the Rat ATPase H+/K+ Exchanging Beta Polypeptide (ATP4B) ELISA Kit provides a reliable tool for studying the role of ATP4B in gastric physiology and pathology, offering researchers the ability to explore new avenues for treatment and management of gastric disorders.

Product Name:Rat ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit
Product Code:RTDL00124
Size:96 Assays
Target:ATP4b
Synonyms:Proton pump beta chain, Gastric H(+)/K(+) ATPase subunit beta
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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