Rat ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
The Rat ATPase Ca++ transporting plasma membrane 2 (ATP2B2) ELISA Kit is a powerful tool for the quantitative measurement of ATP2B2 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ATP2B2 is a critical membrane protein responsible for transporting calcium ions across the plasma membrane, playing a crucial role in cellular calcium homeostasis and signaling. Dysregulation of ATP2B2 has been linked to various diseases, including cardiovascular disorders, neurodegenerative conditions, and cancer.
Monitoring ATP2B2 levels can provide valuable insights into these pathologies and aid in the development of targeted therapies.Overall, the Rat ATPase Ca++ transporting plasma membrane 2 (ATP2B2) ELISA Kit is a valuable tool for researchers studying calcium ion transport mechanisms and their implications in disease processes. Its high performance and versatility make it an essential component of any laboratory focused on understanding the role of ATP2B2 in health and disease.
Product Name:
Rat ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
