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Rat Aspartate Aminotransferase (AST) ELISA Kit

SKU:
RTDL00116
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13221
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
cCAT,AAT,ASAT,SGOT,GOT1,Cysteine transaminase, cytoplasmic,Transaminase A,Aspartate Transaminase 1,Cytoplasmic,Glutamic-Oxaloacetic Transaminase 1,Soluble
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

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Rat Aspartate Aminotransferase (AST) ELISA Kit

The Rat Aspartate Aminotransferase (AST) ELISA Kit is a reliable and accurate assay designed for the quantification of AST levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing precise and consistent results for a variety of research applications.AST is a key enzyme involved in amino acid metabolism and is commonly used as a biomarker for liver and muscle damage. Changes in AST levels can indicate various pathological conditions, making it a valuable tool for monitoring disease progression and assessing treatment efficacy.

With the Rat AST ELISA Kit, researchers can confidently measure AST levels in rat samples, allowing for in-depth research into liver function, muscle health, and disease mechanisms. This kit is essential for studies involving liver diseases, muscle disorders, and drug toxicity, providing valuable insights for both basic research and clinical applications.

Product Name:Rat Aspartate Aminotransferase (AST) ELISA Kit
Product Code:RTDL00116
Size:96 Assays
Target:AST
Synonyms:cCAT, AAT, ASAT, SGOT, GOT1, Cysteine transaminase, cytoplasmic, Transaminase A, Aspartate Transaminase 1,Cytoplasmic, Glutamic-Oxaloacetic Transaminase 1,Soluble
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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