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Rat Aspartate Aminotransferase 2 (AST2) ELISA Kit

SKU:
RTDL00117
Product Type:
ELISA Kit
Sensitivity:
0.107ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GOT2,MitAAT,KATIV,KAT4,FABP-1,Plasma membrane-associated fatty acid-binding protein,Glutamic-Oxaloacetic Transaminase 2,Mitochondrial,Kynurenine Aminotransferase IV
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

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Rat Aspartate Aminotransferase 2 (AST2) ELISA Kit

The Rat Aspartate Aminotransferase 2 (AST2) ELISA Kit is a reliable and accurate tool for detecting levels of AST2 in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it perfect for various research applications.AST2 is an important enzyme involved in amino acid metabolism and liver function. Abnormal levels of AST2 are associated with liver damage, making it a valuable biomarker for studying liver diseases and evaluating potential treatment options.

This ELISA kit is easy to use and offers quick results, allowing researchers to efficiently analyze AST2 levels in rat samples. Overall, the Rat Aspartate Aminotransferase 2 (AST2) ELISA Kit is an indispensable tool for studying liver function and related disorders in rat models. Visit www.assaygenie.com/rat-aspartate-aminotransferase-2-ast2-elisa-kit/ for more information and to order your kit today.

Product Name:Rat Aspartate Aminotransferase 2 (AST2) ELISA Kit
Product Code:RTDL00117
Size:96 Assays
Target:AST2
Synonyms:GOT2, MitAAT, KATIV, KAT4, FABP-1, Plasma membrane-associated fatty acid-binding protein, Glutamic-Oxaloacetic Transaminase 2,Mitochondrial, Kynurenine Aminotransferase IV
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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