Rat ARG1 (Arginase I) ELISA Kit
- SKU:
- AEES00316
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.47 ng/mL
- Range:
- 0.78-50 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat ARG1 (Arginase I) ELISA Kit
The Rat ARG1 (Arginase I) ELISA Kit is a cutting-edge tool meticulously designed for the precise and quantitative detection of Arginase I levels in a variety of rat biological samples. Arginase I, a crucial enzyme involved in the urea cycle, plays a fundamental role in the metabolism of arginine to ornithine and urea, impacting various physiological processes including nitrogen balance and the immune response. Accurate measurement of Arginase I levels using this ELISA kit provides researchers with valuable insights into arginine metabolism, cellular proliferation, immune regulation, and tissue repair pathways where this enzyme plays a pivotal role.
This ELISA kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results vital for investigating the functions and pathways associated with Arginase I. Manufactured under stringent quality control measures, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for researchers aiming to expand their understanding of arginine metabolism and its implications in health and disease. Trust the Rat ARG1 ELISA Kit to enhance your research capabilities and delve deeper into the complexities of metabolic pathways influenced by Arginase I.
| Product Name: | Rat ARG1 (Arginase I) ELISA Kit |
| Product Code: | AEES00316 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.78-50 ng/mL |
| Sensitivity: | 0.47 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat ARG1 in samples. No significant cross-reactivity or interference between Rat ARG1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat ARG1 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ARG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ARG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ARG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ARG1. You can calculate the concentration of Rat ARG1 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ARG1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ARG1 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.72 | 4.19 | 23.12 | 2.48 | 4.09 | 23.01 |
| Standard deviation | 0.14 | 0.21 | 0.81 | 0.17 | 0.22 | 1.08 |
| CV (%) | 5.15 | 5.01 | 3.50 | 6.85 | 5.38 | 4.69 |
Recovery
The recovery of Rat ARG1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-109 | 99 |
| EDTA plasma (n=8) | 86-100 | 91 |
| Cell culture media (n=8) | 95-110 | 101 |
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
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