The Rat ARG (Arginase) CLIA Kit is specifically designed for the quantitative detection of arginase levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.Arginase is an enzyme that plays a crucial role in the urea cycle, converting arginine into ornithine and urea. Dysregulation of arginase has been associated with various diseases, including cardiovascular diseases, liver disorders, and immune-related conditions.
Therefore, measuring arginase levels in rat samples can provide valuable insights into the pathogenesis of these diseases and help in the development of potential therapeutic interventions.Overall, the Rat ARG (Arginase) CLIA Kit is a valuable tool for researchers studying arginase function and its implications in various disease processes in rats. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an essential component for any research laboratory.
Product Name:
Rat Arg (Arginase) CLIA Kit
SKU:
RTES00045
Target:
Rat Arg (Arginase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Arg. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Arg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Arg, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Arg. You can calculate the concentration of Rat Arg in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
92-105
90-105
Average (%)
107
98
98
1:4
Range (%)
100-115
96-110
87-97
Average (%)
108
102
92
1:8
Range (%)
94-109
93-106
100-116
Average (%)
99
100
107
1:16
Range (%)
87-100
87-98
91-103
Average (%)
93
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.28
4.38
0.56
1.36
4.11
Standard deviation
0.06
0.1
0.45
0.07
0.11
0.44
C V (%)
11.32
7.81
10.27
12.5
8.09
10.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Arg concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Arg in samples. No significant cross-reactivity or interference between Rat Arg and analogues was observed.
