The Rat AR (Amphiregulin) CLIA Kit is specifically designed for the precise measurement of amphiregulin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for various research purposes.Amphiregulin is a key signaling protein involved in cell growth, differentiation, and tissue repair. Its dysregulation has been implicated in various diseases, including cancer, inflammatory disorders, and wound healing deficiencies.
Therefore, monitoring amphiregulin levels is crucial for understanding the underlying mechanisms of these conditions and exploring potential therapeutic strategies.With its user-friendly protocol and reliable performance, the Rat AR CLIA Kit is an invaluable tool for scientists and researchers studying the roles of amphiregulin in health and disease. Get your kit today and advance your research in the field of biomarker discovery and therapeutic development.
Product Name:
Rat AR (Amphiregulin) CLIA Kit
SKU:
RTES00352
Target:
Rat AR (Amphiregulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AR. You can calculate the concentration of Rat AR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
91-103
99-115
Average (%)
95
98
105
1:4
Range (%)
103-116
102-119
102-115
Average (%)
109
110
109
1:8
Range (%)
96-109
96-110
95-107
Average (%)
103
102
100
1:16
Range (%)
101-117
88-99
102-119
Average (%)
107
93
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
94-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.79
83.96
384.98
39.36
88.84
368.18
Standard deviation
4.61
9.13
28.6
3.48
10.26
23.27
C V (%)
12.53
10.87
7.43
8.84
11.55
6.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AR in samples. No significant cross-reactivity or interference between Rat AR and analogues was observed.
