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Rat Aquaporin 4 (AQP4) ELISA Kit

SKU:
RTDL00106
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P47863
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HMIWC2,MIWC,WCH4,Mercurial-insensitive water channel
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Aquaporin 4 (AQP4) ELISA Kit

The Rat Aquaporin 4 (AQP4) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of AQP4 in rat samples. This kit offers high sensitivity and specificity, providing dependable and consistent results for a variety of research applications.Aquaporin 4 is a key protein involved in regulating water transport in the brain and spinal cord, playing a crucial role in maintaining brain homeostasis. Abnormalities in AQP4 expression have been linked to neurological conditions such as neuromyelitis optica (NMO) and traumatic brain injury, making it a valuable biomarker for studying these diseases and developing potential treatments.

With its precision and reliability, the Rat AQP4 ELISA Kit is an essential tool for researchers investigating the role of AQP4 in neurological disorders and exploring new therapeutic strategies.

Product Name:Rat Aquaporin 4 (AQP4) ELISA Kit
Product Code:RTDL00106
Size:96 Assays
Target:AQP4
Synonyms:HMIWC2, MIWC, WCH4, Mercurial-insensitive water channel
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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