Rat AQP-4 (Aquaporin 4) ELISA Kit
- SKU:
- AEES00315
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.10 ng/mL
- Range:
- 0.16-10 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat AQP-4 (Aquaporin 4) ELISA Kit
The Rat AQP-4 (Aquaporin 4) ELISA Kit is an advanced assay designed for the precise quantitative assessment of Aquaporin 4 levels in a variety of rat biological samples. Aquaporin 4. as a prominent member of the aquaporin family, plays a critical role in regulating water permeability in cell membranes. By accurately measuring Aquaporin 4, researchers can gain valuable insights into fluid balance, cell volume regulation, and the physiological processes modulated by this essential water channel protein. This ELISA kit guarantees exceptional sensitivity and specificity, ensuring accurate and reproducible results crucial for investigating the functions and pathways associated with Aquaporin 4.
Manufactured under strict quality control standards, this kit offers robust performance and user-friendly protocols, making it an optimal choice for researchers looking to delve deeper into the mechanisms of water transport and cellular homeostasis. Trust the Rat AQP-4 ELISA Kit to elevate your research capabilities and expand your understanding of water channel biology and its implications in health and disease.
| Product Name: | Rat AQP-4 (Aquaporin 4) ELISA Kit |
| Product Code: | AEES00315 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat AQP-4 in samples. No significant cross-reactivity or interference between Rat AQP-4 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat AQP-4 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AQP-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AQP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AQP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AQP-4. You can calculate the concentration of Rat AQP-4 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
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