The Rat AQP-4 (Aquaporin-4) CLIA Kit is a reliable and accurate tool for measuring levels of AQP-4 in rat serum, plasma, and tissue culture samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Aquaporin-4 is a key protein that plays a critical role in regulating water movement in the central nervous system, particularly in the brain and spinal cord. Dysregulation of AQP-4 has been linked to various neurological disorders such as multiple sclerosis, neuromyelitis optica, and brain edema, highlighting its importance as a potential biomarker for these conditions.
With its advanced technology and user-friendly design, the Rat AQP-4 CLIA Kit is a valuable resource for researchers investigating the role of AQP-4 in neurological diseases and exploring potential therapeutic strategies.
Product Name:
Rat AQP-4 (Aquaporin 4) CLIA Kit
SKU:
RTES00044
Target:
Rat AQP-4 (Aquaporin 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AQP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AQP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AQP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AQP-4. You can calculate the concentration of Rat AQP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
87-100
102-119
Average (%)
107
93
109
1:4
Range (%)
92-107
93-106
101-114
Average (%)
100
99
107
1:8
Range (%)
87-101
102-115
91-102
Average (%)
94
109
97
1:16
Range (%)
86-101
96-110
97-109
Average (%)
93
104
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.93
260.12
787.27
119.88
271.23
864.65
Standard deviation
13.21
19.3
64.01
12.35
32.36
75.66
C V (%)
12.02
7.42
8.13
10.3
11.93
8.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AQP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AQP-4 in samples. No significant cross-reactivity or interference between Rat AQP-4 and analogues was observed.
