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Rat Apolipoprotein A4 (APOA4) ELISA Kit

SKU:
RTDL00093
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02651
Sensitivity:
27ng/mL
Range:
78.12-5000ng/mL
ELISA Type:
Sandwich
Synonyms:
ApoA-IV,ApoAIV,Apo-A4
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Apolipoprotein A4 (APOA4) ELISA Kit

The Rat Apolipoprotein A4 (APOA4) ELISA Kit is a reliable and accurate tool for the quantitative measurement of APOA4 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, allowing for precise and reproducible results in various research applications.APOA4 is a key protein that plays a crucial role in lipid metabolism and cardiovascular health.

It is known to be involved in lipid transport and metabolism, making it a valuable biomarker for studying conditions such as obesity, cardiovascular diseases, and metabolic disorders. The Rat APOA4 ELISA Kit provides researchers with a valuable tool for investigating the role of APOA4 in these conditions, aiding in the development of potential therapies and interventions.

Product Name:Rat Apolipoprotein A4 (APOA4) ELISA Kit
Product Code:RTDL00093
Size:96 Assays
Target:APOA4
Synonyms:ApoA-IV, ApoAIV, Apo-A4
Detection Method:Sandwich
Range:78.12-5000ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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