The Rat APOC3 (Apolipoprotein C3) ELISA Kit is specifically designed for the accurate measurement of apolipoprotein C3 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Apolipoprotein C3 is a key protein involved in lipid metabolism and has been linked to conditions such as hypertriglyceridemia, obesity, and cardiovascular diseases.
Understanding the levels of APOC3 in biological samples can provide valuable insights into the mechanisms underlying these disorders and aid in the development of targeted therapies.With easy-to-follow protocols and a user-friendly format, the Rat APOC3 ELISA Kit is an essential tool for researchers studying lipid metabolism, cardiovascular health, and related diseases in rat models. Trust Assay Genie for accurate and insightful results in your research endeavors.
Product Name:
Rat ApoC3 (Apolipoprotein C3) ELISA Kit
SKU:
RTES01015
Target:
Rat ApoC3 (Apolipoprotein C3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/ml
Detection range:
6.25-400 ng/ml
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoC3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoC3. You can calculate the concentration of Rat ApoC3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
92-103
96-110
Average (%)
97
98
102
1:4
Range (%)
97-111
87-98
93-105
Average (%)
102
93
99
1:8
Range (%)
95-111
82-95
92-108
Average (%)
103
87
98
1:16
Range (%)
100-114
84-95
99-114
Average (%)
107
89
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.7
61.0
184.9
19.2
59.0
192.0
Standard deviation
1.2
3.5
9.4
1.1
2.5
6.0
C V (%)
5.8
5.74
5.08
5.73
4.24
3.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ApoC3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoC3 in samples. No significant cross-reactivity or interference between Rat ApoC3 and analogues was observed.
