The Rat APOA2 (Apolipoprotein A2) CLIA Kit is specifically designed for the accurate measurement of apolipoprotein A2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Apolipoprotein A2 is an important protein involved in lipid metabolism and plays a key role in regulating cholesterol levels in the body.
Abnormalities in APOA2 levels have been linked to cardiovascular diseases and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its reliable performance and ease of use, the Rat APOA2 CLIA Kit is a valuable tool for researchers studying lipid metabolism, cardiovascular health, and metabolic diseases in rat models.
Product Name:
Rat ApoA2 (Apolipoprotein A2) CLIA Kit
SKU:
RTES00614
Target:
Rat ApoA2 (Apolipoprotein A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ApoA2. You can calculate the concentration of Rat ApoA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
86-100
97-111
Average (%)
98
93
104
1:4
Range (%)
90-104
96-109
100-115
Average (%)
97
102
106
1:8
Range (%)
94-108
98-110
86-102
Average (%)
101
105
93
1:16
Range (%)
91-101
95-109
87-102
Average (%)
96
103
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.32
9.5
28.49
4.24
9.55
31.22
Standard deviation
0.52
0.83
2.96
0.44
1.0
3.29
C V (%)
12.04
8.74
10.39
10.38
10.47
10.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ApoA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoA2 in samples. No significant cross-reactivity or interference between Rat ApoA2 and analogues was observed.
