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Rat Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit

SKU:
RTDL00080
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R560
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CARP,ALRP,C-193,CVARP,MCARP,Ankyrin Repeat Domain 1(Cardiac Muscle),Cardiac ankyrin repeat protein,Cytokine-inducible gene C-193,Cytokine-inducible nuclear protein
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Rat Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit

The Rat ANKRD1 (Ankyrin Repeat Domain Protein 1) ELISA Kit is specifically designed for the accurate quantification of ANKRD1 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reliable results in various research applications.ANKRD1 is a key protein involved in cellular processes such as gene expression, cell differentiation, and muscle development. Dysregulation of ANKRD1 has been linked to various diseases, including cardiovascular disorders, muscular dystrophy, and cancer.

Therefore, accurate measurement of ANKRD1 levels is essential for understanding its role in disease progression and potential therapeutic interventions.Overall, the Rat ANKRD1 ELISA Kit provides researchers with a valuable tool for studying the functions of ANKRD1 in biological processes and disease pathways, ultimately contributing to advancements in medical research and drug development.

Product Name:Rat Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit
Product Code:RTDL00080
Size:96 Assays
Target:ANKRD1
Synonyms:CARP, ALRP, C-193, CVARP, MCARP, Ankyrin Repeat Domain 1(Cardiac Muscle), Cardiac ankyrin repeat protein, Cytokine-inducible gene C-193, Cytokine-inducible nuclear protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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