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Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

SKU:
RTDL00044
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25095
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AT1R,AG2S,AG2-S,AGTR1A,AGTR1B,AT1,AT1B,AT2R1,AT2-R1,AT2R1A,AT2R1B,HAT1R,AT1 Receptor,Angiotensin Receptor 1B
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

The Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit is a valuable tool for accurate measurement of AGTR1 levels in rat serum, plasma, and tissue samples. This kit is characterized by high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes.AGTR1 is a key receptor involved in the renin-angiotensin system, playing a critical role in regulating blood pressure, fluid balance, and vascular tone. Dysregulation of AGTR1 has been implicated in conditions such as hypertension, heart failure, and kidney disease, making it a valuable biomarker for studying these disorders and developing potential therapeutic interventions.

By utilizing the Rat AGTR1 ELISA Kit, researchers can gain valuable insights into the role of AGTR1 in health and disease, leading to a better understanding of cardiovascular pathophysiology and potential avenues for targeted treatment strategies.

Product Name:Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit
Product Code:RTDL00044
Size:96 Assays
Target:AGTR1
Synonyms:AT1R, AG2S, AG2-S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2-R1, AT2R1A, AT2R1B, HAT1R, AT1 Receptor, Angiotensin Receptor 1B
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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